Community analyses of sulfidogenic 2-bromophenol-dehalogenating and phenol-degrading microbial consortia

  Microbial consortia were accomplished beneath sulfidogenic altitude application a accepted estuarine debris inoculum with 2-bromophenol or phenol as the sole the carbon source. Stable consortia were maintained over a 3-year aeon with again agriculture and consecutive concoction into beginning medium. 2-Bromophenol was initially dehalogenated to phenol.
  Degradation of phenol was abased on sulfate abridgement and inhibited by molybdate, a specific inhibitor of sulfate reduction. Reductive dehalogenation of 2-bromophenol, however, was not abased on, or inhibited by sulfate. The 2-bromophenol- and phenol-degrading sulfidogenic consortia were characterized application 16S rRNA brake fragment breadth polymorphism assay and different clones were sequenced. Terminal brake fragment breadth polymorphism of all alone clones and both microbial consortia adumbrated that all 16S rRNA types present in the consortia were cloned and characterized.
  Four phylotypes were articular from the 2-bromophenol-utilizing bunch which based aloft their 16S rRNA sequences amassed into three above groups: one arrangement was accompanying to the ϵ-subgroup of the Proteobacteria, two clones amassed aural the sulfate-reducers (δ-subgroup of Proteobacteria), the fourth phylotype was aberrant from ahead declared bacilli and was a lot of carefully accompanying to the brand Planctomycetes.